Presence of Subgenomic mRNAs in Virions of Coronavirus IBV
Identifieur interne : 001338 ( Main/Exploration ); précédent : 001337; suivant : 001339Presence of Subgenomic mRNAs in Virions of Coronavirus IBV
Auteurs : X. Zhao [Royaume-Uni] ; K. Shaw [Royaume-Uni] ; D. Cavanagh [Royaume-Uni]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: The presence of subgenomic mRNAs in virions of IBV was examined by probing Northern blots of RNA extracted from virions using as a probe a cDNA of the 3′-terminal nucleocapsid protein (N) gene. This detects all five mRNAs because of the 3′-coterminal, nested-set arrangement of coronavirus mRNAs. The mRNAs were readily detected even after extensive purification of virions and after RNase A treatment of virions. In sucrose gradients the peaks of virus particles, genomic RNA (gRNA), and mRNAs were coincident. Cellular mRNA was not detected in virions. The molar ratio of gRNA to each mRNA ranged from about 10 to 30 for IBV-Beaudette and 25 to 800 for IBV-M41. The molar ratio of genomic to intracellular viral mRNAs was also determined. From this it was estimated that the efficiency of incorporation of gRNA into virions was at least 20- to 100-fold greater, depending on the mRNA species, for IBV-Beaudette and 100- to 500-fold for IBV-M41. It is concluded that most virions contain only gRNA or contain only one species of mRNA on average in addition to gRNA.
Url:
DOI: 10.1006/viro.1993.1465
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000357
- to stream Istex, to step Curation: 000337
- to stream Istex, to step Checkpoint: 000503
- to stream Main, to step Merge: 001354
- to stream Main, to step Curation: 001338
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Presence of Subgenomic mRNAs in Virions of Coronavirus IBV</title><author><name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X." last="Zhao">X. Zhao</name></author><author><name sortKey="Shaw, K" sort="Shaw, K" uniqKey="Shaw K" first="K." last="Shaw">K. Shaw</name></author><author><name sortKey="Cavanagh, D" sort="Cavanagh, D" uniqKey="Cavanagh D" first="D." last="Cavanagh">D. Cavanagh</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C08406B6F69450EB9D703FB00548D617647BB3F6</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/viro.1993.1465</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-ZQL3DMPV-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000357</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000357</idno><idno type="wicri:Area/Istex/Curation">000337</idno><idno type="wicri:Area/Istex/Checkpoint">000503</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000503</idno><idno type="wicri:doubleKey">0042-6822:1993:Zhao X:presence:of:subgenomic</idno><idno type="wicri:Area/Main/Merge">001354</idno><idno type="wicri:Area/Main/Curation">001338</idno><idno type="wicri:Area/Main/Exploration">001338</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Presence of Subgenomic mRNAs in Virions of Coronavirus IBV</title><author><name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X." last="Zhao">X. Zhao</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG16 0NN</wicri:regionArea><wicri:noRegion>Berkshire RG16 0NN</wicri:noRegion></affiliation></author><author><name sortKey="Shaw, K" sort="Shaw, K" uniqKey="Shaw K" first="K." last="Shaw">K. Shaw</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG16 0NN</wicri:regionArea><wicri:noRegion>Berkshire RG16 0NN</wicri:noRegion></affiliation></author><author><name sortKey="Cavanagh, D" sort="Cavanagh, D" uniqKey="Cavanagh D" first="D." last="Cavanagh">D. Cavanagh</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>AFRC Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG16 0NN</wicri:regionArea><wicri:noRegion>Berkshire RG16 0NN</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">196</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="172">172</biblScope><biblScope unit="page" to="178">178</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The presence of subgenomic mRNAs in virions of IBV was examined by probing Northern blots of RNA extracted from virions using as a probe a cDNA of the 3′-terminal nucleocapsid protein (N) gene. This detects all five mRNAs because of the 3′-coterminal, nested-set arrangement of coronavirus mRNAs. The mRNAs were readily detected even after extensive purification of virions and after RNase A treatment of virions. In sucrose gradients the peaks of virus particles, genomic RNA (gRNA), and mRNAs were coincident. Cellular mRNA was not detected in virions. The molar ratio of gRNA to each mRNA ranged from about 10 to 30 for IBV-Beaudette and 25 to 800 for IBV-M41. The molar ratio of genomic to intracellular viral mRNAs was also determined. From this it was estimated that the efficiency of incorporation of gRNA into virions was at least 20- to 100-fold greater, depending on the mRNA species, for IBV-Beaudette and 100- to 500-fold for IBV-M41. It is concluded that most virions contain only gRNA or contain only one species of mRNA on average in addition to gRNA.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X." last="Zhao">X. Zhao</name></noRegion><name sortKey="Cavanagh, D" sort="Cavanagh, D" uniqKey="Cavanagh D" first="D." last="Cavanagh">D. Cavanagh</name><name sortKey="Shaw, K" sort="Shaw, K" uniqKey="Shaw K" first="K." last="Shaw">K. Shaw</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001338 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001338 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:C08406B6F69450EB9D703FB00548D617647BB3F6
|texte= Presence of Subgenomic mRNAs in Virions of Coronavirus IBV
}}
| This area was generated with Dilib version V0.6.33. |  |